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Increase of resting Ca 2+ levels and amplitude of vasopressin-induced Ca 2+ transients were observed when cells in serum-free medium were exposed to 5mM Ca 2+ for 2h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10mM Ca 2+ and absence of serum. However, cells exposed to 10mM Ca 2+ in the presence...
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