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Background and ObjectivesDifferentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. This is particularly relevant when bacteria have been exposed to antimicrobial agents. The objective of this study was to test a method using quantitative real‐time polymerase chain reaction (qPCR) combined with propidium monoazide (PMA), developed...
Background and ObjectivesOne of the major disadvantages of DNA‐based microbial diagnostics is their inability to differentiate DNA between viable and dead microorganisms, which could be important when studying etiologically relevant pathogens. The aim of this investigation was to optimize a method for the selective detection and quantification of only viable Aggregatibacter actinomycetemcomitans and...
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