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Equilibrium constants for the association of human protein translation initiation factor eIF4E with two mRNA 5′-cap analogs, namely 7-methylguanosine 5′-triphosphate and P 1 -(7-methylguanosine-5′) P 3 -(guanosine-5′) triphosphate, and with guanosine 5′-monophosphate have been redetermined by the fluorescence quenching method taking the inner filter effect of the cap-analog into account...
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