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We report a spheroid forming lab-on-a-chip that can be used to control the size of three-dimensional hepatocyte spheroids by using micro-rotational flow. This device can form hepatic spheroids with diameters in the range 130-430 ??m by varying the chamber diameter and the cell density. The developed lab-on-a-chip consisted of a polydimethylsiloxane-based micro-chamber produced by soft lithography...
Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The...
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