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W pracy przedstawiono przegląd metod analizy ekspresji genów z zastosowaniem technik: mikromacierzy DNA, seryjnej analizy ekspresji genów (SAGE), jak również odwrotnej transkrypcji – RT-PCR oraz qRT-PCR. Zaprezentowano możliwości wykorzystania poszczególnych technik wraz z przykładami ich praktycznego zastosowania w badaniach molekularnych rzepaku.
The aim of the presented work was the functional comparison of conventional 21-day-old and modified 6-day-old Caco-2 cell cultures for their application in studies on the bacterial adhesion and transepithelial transport. In the short-term culture system, the cells were grown on polycarbonate filters in serum reduced medium supplemented with a combination of growth factors. Morphological comparison...
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