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The objective of the study was to investigate the effects of an in ovo injection of CuNano and the timing of injection on metabolic rate (O 2 consumption and heat production, HP) and development of layer hatchlings. On day 1 of incubation, 192 fertile eggs from 29-week-old Lohmann breeder strain chickens were distributed into four groups that were administered colloidal CuNano on: day 1 and/or...
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