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This paper reports on the elucidation of the possible mechanism for forming the putty structure of hyaluronan (HA) at low pH (2.5) using rheological methods. Oscillation and stress relaxation tests on a series HA putty samples containing various concentrations of salt indicated that ionic forces were not the driving force for their formation, but hydrogen bonds were responsible for driving two or...
A new method was developed for quantitative determination of the viscoelastical characteristics of hyaluronic acid (HA) putty (formed at pH 2.5) using a stress relaxation approach. A concept of entanglement network numbers (ENN) was applied to provide a quantitative measure for the strength of HA putty. Based on the new approach, the relationship between ENN and HA concentration was established. The...
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