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A new homogeneous fluoroimmunoassay method based on the use of dynamic long-wavelength fluorescence polarization is presented here for the first time. This methodology, which is applied to the determination of linear alkylbenzenesulfonates (LASs) in water samples, involves the use of a new long-wavelength tracer synthesized from the oxazine dye Nile Blue (NB) via a carbodiimide method. This tracer...
A homogeneous competitive immunoassay for gliadin determination is described for the first time. Gliadins are labeled with a terbium(III) chelate to form the tracer which competes with unlabeled gliadins for the active sites of anti-gliadin antibodies. The terbium(III) is released from the tracer by means of an “acidic enhancement solution” and the initial rate of the dissociation reaction is monitored...
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