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Studied was the activity of selected degradative enzymes of lysosomal, microsomal and cytoplasmic fraction of hepatocytes as affected by different protein levels in the diet of experimental mice. The control animals were fed with standard 16% protein level in the diet, while experimental animals with low (10%) or high (40%) protein diet. The animals were fed ad libitum. Changes in fractions depended...
The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experimental groups (A-F) with 250 μl 0.9% NaCl solution containing 20 or 30 mg morphine hydrochloride per kg body weight. The injections were given intramuscularly once a day between 9:00-10:00...
Used were ninety Swiss mice males divided into 9 groups (n=10). Three control groups [I, II, III] were injected with 0.9% NaCl solution, while six experimental groups (A, B, C, D, E, F) with the morphine hydrochloride. Mice from groups A, C and E were injected with a dose of 20 and those from B, D and F with a dose of 30 mg morphine per kg body weight. In each group, both solutions were administered...
The experiment was carried out on 20 Swiss male mice divided into experimental (E, n=10) and control (C, n=10) group. E mice were intraperitoneally injected with glucagon (15 μg/kg b.w.) twice daily for eight days, while mice C with 250 μl 0.9% NaCl/mouse (to exclude the injection effect only).In the lysosomal fraction of the liver the activities of acid phosphatase, β-N-acetyl-hexosaminidase, β-glucuronidase,...
The investigations were conducted on 20 Swiss male mice, fed a feed containing 16% (control group,n=10) or 10% protein (experimental group, n=10). After 14 days of feeding, sections of liver and kidneys were obtained from all animals and homogenized in order to obtain the lysosomal fraction,in which the activity of the following enzymes was determined: acid phosphatase, lysosomal esterase,lysosomal...
The study was conducted on 20 Swiss line male mice divided into two groups – control (n=10) comprised of unselected animals (group C), and experimental (n=10) comprised of mice selected over 24 generations for high live body weight gain (group S). Beginning from day 42 of life mice of both groups received over a period of 14 days a standard pelleted feed containing 16% protein.After 14 days the animals...
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