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Currently, there is an urgent need to develop genetically engineered multivalent subunit vaccines or DNA vaccines to provide protection against infection of multiple types of Clostridium perfringens. In this study, we expressed the fusion protein of α-β2-ε-toxin derived from C. perfringens in Escherichia coli. For the first time, an efficient expression of a soluble α-β2-ε-toxin in E. coli was obtained...
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