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A novel mutation, insertion/deletion (InDel) was found in the porcine prolactin gene exon 5 coding for the 3’UTR. Polymorphism was detected and identified using PCR-MSSCP and DNA sequencing techniques, and then analysed with PCR-RFLP using HpyCH4III endonuclease. Inserted/deleted was an 11-bp TGTTTCTTAAC motive that occurred once or twice at position 201-222 in the amplified DNA fragment. The investigations...
The MyoD family genes were considered as candidate genes for growth rate and carcass meatiness in pigs. Gene mutations (SNPs, single nucleotide polymorphisms) discovered in the coding and 5’-flanking regions of the MYOD1 and MYF5 were analysed as possible causal mutations for these traits. Studies of the relation between polymorphisms in both those genes and productive traits were performed on Polish...
The aim of the study was to characterize the polymorphism of calpastatin (CAST) gene identified with three restriction enzymes (HinfI, MspI, RsaI) in several pig breeds and lines bred in Poland and to evaluate the relation between the CAST genotypes and carcass traits. The analyses covered a total of 294 fatteners of Polish Landrace (PL), Pietrain (P), Zlotnicka Spotted (ZS), Torhyb [P × (Polish Large...
The aim of this study was to characterize the polymorphism of GH and LEP genes in selected pig breeds reared in Poland and to analyse the relation between the GH and LEP genotypes and carcass meat and fat deposition. The tests covered a total of 305 animals of the following breeds and lines:Pietrain (P), Złotnicka Spotted (ZS), Polish Landrace (PL), Torhyb [P × (PL × Polish Large White)],Stamboek...
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