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A recombinant plasmid pETD-SL was constructed to analyze the effect of hydrogen production by [NiFe] hydrogenase gene isolated from Rhodopeseudomonas palustris. A two-promoter vector pETDuet-1 was used to construct pETD-SL which contains hupS and hupL gene, and then pETD-SL was transferred into recombinant Escherichia coli BL21(DE3) which was named E. coli BH20. Furthermore, HupS and HupL protein...
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