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The aim of the study was to use real time RT-PCR for the detection of genetic material of bovine viral diarrhea virus (BVDV) type 1 and type 3 in serum and milk samples. Material tested included the fetal calf serum used for cell culture, serum samples from healthy calves and from calves experimentally inoculated with BVDV type 1 and type 3, and milk samples (pasteurized and treated with ultra high...
Twelve batches of commercially available foetal bovine sera were tested for the presence of bovine viral diarrhoea virus (BVDV) and BDVD antibodies. The antibodies were found in three batches coming from two suppliers. Fragments of BVDV genome were present in five of these batches when tested by RT-PCR. All of them were type 1 BVDV according to a direct sequencing of PCR products. However, the further...
The aim of the study was to identify, using serological and virological tests coupled with RT-PCR, animals in a herd suspected of infection with bovine viral diarrhea virus (BVDV). All the animals, except two, were seropositive for BVDV in a group of 26 herd-mates. One animal showing a slow growth rate and pulmonary infection was suggested to be possible persistently infected with the virus. Three...
Contemporary views on molecular biology of bovine viral diarrhea virus are reviewed. Diversity of viral genome in the region coding for p125/NS23 and functions of viral proteins are described. Special emphasis is put on viral cytopathogenicity correlated with the rearrangement of nucleotide sequences of cytopathic strains of BVDV in a region coding for p125/NS23 protein. Pathogenesis of Mucosal Disease...
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