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A rapid liquid chromatography tandem mass spectrometry method was developed and validated to detect and confirm five thyreostatic drugs: tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil in animal feeding stuff samples. Thyreostats were extracted from feed with methanol, and then degreasing of the extract with petroleum ether was performed, followed by the derivatisation...
A simple and sensitive gas chromatography method was developed to determine a group of oestrogens in surface water. In the first stage of analysis, enzymatic hydrolysis of oestrogen metabolites with glucuronidase AS-HP was performed. Free compounds were extracted from 200 mL of water sample on C18 SPE column (6 mL, 1000 mg). The evaporated extract was subjected to derivatisation with a mixture of...
Sensitive and selective methods for the screening (GC-MS) and confirmatory analysis (GC-MS/MS) of 17α- and 17ß- trenbolone in bovine urine were developed. In the first stage of the analysis, the enzymatic hydrolysis of trenbolone metabolites with glucuronidase AS-HP in acetate buffer (pH 5.2) solution was carried out. Free compounds were extracted from urine with diethyl ether. For the purification...
To select appropriate diet for hamsters used in the uterotrophic and Hershberger assays two rodent diets were compared: Murigran (Agropol, Poland) and Altromin 7010 (Altromin Spezialfutter GmbH&Co., Germany). The contents of bioactive compounds in feeds were evaluated by liquid chromatography, and their oestrogenic activity by yeast enhanced green fluorescent protein assay. In opposition to Altromin,...
Natural occurrence of thiouracil in bovine and swine urine in Poland was investigated. Under the national residue control programme, 537 urine samples were tested. In 77 samples (14.3%) thiouracil was detected above decision limit CCα (0.91 µg L⁻¹), including eight samples over the recommended concentration of 10 µg L⁻¹. Of the bovine urine samples, 95 and 99 percentiles have thiouracil concentration...
This paper describes the quantitative method of determination of chosen substances from resorcylic acid lactones group: zeranol, taleranol, α-zearalenol, ß-zearalenol, and zearalanone in bovine muscle tissue. The presented method is based on double diethyl ether liquid-liquid extraction (LLE), solid phase extraction (SPE) clean up, and gas chromatography mass spectrometry (GC- MS) analysis. The residues...
A simple and sensitive method was developed for determination of 17β-oestradiol and testosterone in bovine serum. The hormones were analysed and quantified as heptafluorobutyric anhydride derivatives by gas chromatography-mass spectrometry after extraction from serum without any cleanup step. The method was validated according to the requirements listed in the Commission Decision 2002/657/EC. Decision...
Studies on effects of pasteurisation of meat with spiked hormones at residue levels have been made. Beef and poultry meat samples which contained diethylstilbestrol and 19-nortestosterone were heated at 80°C for 20 minutes. The hormones were quantified by high-performance liquid chromatography with UV detection on raw and pasteurised meat samples. Diethylstilbestrol is stable under these conditions...
Studies on the effects of freezing meat with spiked hormones at residue levels have been made. Beef and poultry meat samples which contained diethylstilbestrol and 19-nortestosterone were stored frozen at -20°C. The hormones were quantified by high-performance liquid chromatography with UV detection after 2, 4 and 6 months. Diethylstilbestrol is stable under all of these conditions and shows only...
A simple method for determination of 8 anabolie hormone residues in the muscles and urine of slaughter animals was elaborated. Forty cattle muscle samples and 30 urine samples fortified with diethyl stilbestrol, dienstrol, hexastrol, zeranol, trenbolone, trenbolone acetate, 19-nortesterone, and medroxyprogesterone acetale at concentrations of 1 to 20 ng/g (ppb) were examined. The hormones were extracted...
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