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Thirty-three type A strains of G. abietina from diseased shoots or needles of P. sylvestris, P. nigra and P. armandii and three strains of Brunchorstia pinea var. cembrae from P. mugo were isolated from four regions of Poland differing with respect to climatic conditions. Genetic polymorphism of the mitochondrial small subunit rRNA (mtSSU rRNA), ribosomal RNA fragment including ITS1, 5.8S and ITS2...
On the basis of morphological features and RAPD markers the strains of Chalara ovoidea found in Poland on planks and on stems of beech trees were identified. As reference strains the cultures taken from CBS Utrecht were employed; they were cultures CBS 354.76 and CBS 136.88. The amplification of genomic DNA was conducted using 10 primers (OPA01-OPA10), 7 of which (OPA01-OPA05, OPA09, OPA10) gave positive...
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