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Two different communities of microorganisms were identified in soils by application of the classical method of fungi isolation (soil dilution, culturing on artificial media, morphotyping) and a molecular method (extraction of the environmental DNA, amplification with universal primers NS1 and NS2, cloning and sequencing of representative clones). No organisms were common to both communities. Apart...
The aim of the studies was to check the usefulness of ITS1/2 rDNA and 18S rDNA regions in the molecular investigation of forest soil microbiota structure. Soil studied, originated from a 1−year−old plantation and a 40−year old stand of Scots pine located in Bierzwnik and Międzychów forest districts located 200 km apart. The hypothesis assumed that both approaches lead to the discovery of abundant...
Effects of pre−planting soil preparation on the clear−cut on the community structure of soil fungi and bacteria, their possible biological activity towards Armillaria and Heterobasidion, and mortality of Scots pine trees were studied in 40−year−old Scots pine plantation in Międzychód Forest District (W Poland). Pre−planting soil preparation included: (i) deep ploughing, (ii) shallow furrowing, (iii)...
Effects of post−harvest wood−debris utilization and pre−planting soil preparation in clear−cut forest on the community structure of soil fungi and bacteria and their possible biological activity towards Armillaria and Heterobasidion were studied in 1− and 10−year−old Scots pine plantations in Bierzwnik and Międzychód Forest Districts (W Poland). Post−harvest wood−debris utilization included: (i) removal...
Phytophthora and Pythium species (Oomycota) are known to be serious pathogens of forest trees. Little is known, however, about their presence in Polish oak forests and their role in the oak decline, especially in the flooded areas. The aim of this study was (1) to detect and compare populations of microorganisms from Oomycota and fungi in roots and soil of healthy and declining pedunculate 30−126...
The presence of microorganisms in the substrate is detected with increasing frequency using chemical methods. During the first 9 weeks ergosterol concentration in sterile or not sterile soil inoculated with one of the damping−off fungi reached 966 µg/g, while number of colony forming units (cfu) went up to 247×103 cfu/g. Ergosterol concentration was correlated with the size of the soil fungal community...
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