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Transcriptional activation of bacteriophage T4 middle promoters requires σ 70 -containing Escherichia coli RNA polymerase, the T4 activator MotA, and the T4 co-activator AsiA. T4 middle promoters contain the σ 70 –10 DNA element. However, these promoters lack the σ 70 –35 element, having instead a MotA box centered at –30, which is bound by MotA. Previous work has indicated...
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