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The development and application of bioorthogonal two-step labeling techniques receives much attention. Employing bifunctional proteasome probe 2 the efficiency of two-step labeling of recently published biotinylated cyclooctynes 3–5 is compared to Staudinger–Bertozzi ligation in cell extracts and living cells. While cyclooctynes 3–5 react faster and at a much lower concentration then the Staudinger–Bertozzi...
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