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This research reports a method to fabricate a loop-shaped vessel-like channel for perfusion of culture medium in a cell-laden collagen gel. We used alginate fibers as sacrificial structures for construction of loop-shaped vessel-like channels. By integration of a loop-shaped alginate fiber with connection ports in a cell-laden collagen gel, we formed a tube-connectable loop-shaped channel. Finally,...
This paper describes a core-shell hydrogel microfiber consisted of alginate and tetra-arm poly(ethylene glycol) (tetra-PEG) encapsulating rat insulinoma cells using a microfluidic process with a coaxial device and rapid gelation induced by diffusion of two different cross-linkers of barium ion and dithiothreitol (DTT) in the laminar flow (Fig. 1). Microfluidic techniques are able to encapsulate the...
This paper describes the construction and differentiation of human adipose-derived stem cell (ADSC) fibers into the adipocyte lineage for breast reconstruction. Human ADSCs were encapsulated into the core region of hydrogel core-shell fibers by the cell fiber technology [1], induced for adipogenic differentiation, and maintained for over 2 months. After adipogenic induction, accumulation of lipid...
This paper presents a new method to fabricate 2-dimensional collagen sheets whose internal molecular structures form highly-aligned nanofibers in one direction. In this work, collagen nanofibers are self-aligned in fully 2-dimensional substrates by applying cyclic stretch during the gelation process of collagen solution, and the fabricated collagen sheets induce the alignment of cells without any...
We propose a method for constructing fiber-type three-dimensional (3D) tissue of human iPS-derived cardiomyocytes and quantifying its contractile force in response to the addition of drug. By culturing the cardiomyocytes in micropatterned hydrogel with anchors, we succeeded in fabrication of the fibers with aligned cardiomyocytes and fixation of the fiber edges to the anchors. Since the fiber generated...
This paper describes the self-assembly of “cell springs” using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers...
This paper describes preliminary result of a specially patterned and aligned neural bundle formed by neural stem cell (NSC) microfibers for neural transplantation. The NSC microfibers are made by core-shell hydrogel microfiber encapsulating mouse NSCs. We developed a technique to align microfibers in a straight line and arrange the fibers in special patterns. Using this technique, we formed a neural...
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