The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
This research reports a method to fabricate a loop-shaped vessel-like channel for perfusion of culture medium in a cell-laden collagen gel. We used alginate fibers as sacrificial structures for construction of loop-shaped vessel-like channels. By integration of a loop-shaped alginate fiber with connection ports in a cell-laden collagen gel, we formed a tube-connectable loop-shaped channel. Finally,...
A rapid and high-resolution bioprinter based on a capillary coaxial microfluidic printer head and vacuum substrate, was conceived and built up in this work. We demonstrate the printing of porous (400 μm pitch) 3D bio-scaffolds made from calcium alginate microfibers with a printing resolution (i.e. fiber diameter) of ca. 150 microns, at a speed of 40 mm/s, which is approximately 10x faster than existing...
This paper reports on a biomimetic microskeleton actuated by muscle fibers (figure 1(a)). The 3D microskeleton is based on concatenated rib elements fabricated by two-photon polymerization of a custom-made hydrogel (figure 1(b)). For the first time, micrometer-sized skeletons were fabricated, overgrown with functional muscle fibers, and contracted at significant amplitudes. Compared to planar biomimetic...
This paper describes the construction and differentiation of human adipose-derived stem cell (ADSC) fibers into the adipocyte lineage for breast reconstruction. Human ADSCs were encapsulated into the core region of hydrogel core-shell fibers by the cell fiber technology [1], induced for adipogenic differentiation, and maintained for over 2 months. After adipogenic induction, accumulation of lipid...
This conference proceeding describes a method to construct a skin-equivalent cultured on a curved surface. The skin-equivalent consists of not only the dermis/epidermis but also perfusable channels. We embedded an anchoring structure in a culture device to prevent the horizontal contraction of the tissue during cultivation. Owing to perfusion of culture medium via the channels, we can culture the...
This paper reports the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfibers; the core consists of iPSCs with or without extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. We revealed that mouse iPSCs cultured in the micro-scale space with ECM proteins sustain their pluriotency efficiently. This 3D culture system...
We propose a method for constructing fiber-type three-dimensional (3D) tissue of human iPS-derived cardiomyocytes and quantifying its contractile force in response to the addition of drug. By culturing the cardiomyocytes in micropatterned hydrogel with anchors, we succeeded in fabrication of the fibers with aligned cardiomyocytes and fixation of the fiber edges to the anchors. Since the fiber generated...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.