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We demonstrate a novel biosensor for in vitro observation of cell membrane behaviors associated with proteins, adapting a supported lipid bilayer (SLB) that mimics the cellular membrane and using label-free surface plasmon resonance spectroscopy (SPRS). In this study, we focus on the detection of membrane behaviors by the specific types of Cu/Zn-superoxide dismutase (SOD1) species, which have been...
Specific interactions between heavy metal ions and biomaterials have advantages in the sensitive and selective detection of the metal ions. In this study, Cu-demetallated form of Cu/Zn-superoxide dismutase (SOD1) protein was applied as a sensing probe in order to detect Cu(II) ions selectively via surface plasmon resonance (SPR) analysis. Because demetallated metalloproteins have vacant specific metal-binding...
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