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Synthetic and genetically encoded optical switch probes and lock-in detection methods provide a means to modulate and isolate fluorescence signals from the optical switch and protein complexes of interest against un-modulated background sources. In this paper we propose an improved method for optical lock-in detection (OLID) fluorescence microscopy that can significantly enhance image contrast of...
In this paper, a segmentation scheme that combines thresholding, morphological operation and region growing is proposed for tracing the vocal fold opening region from the high-speed laryngeal image sequences. Specifically, the proposed scheme contains three steps: 1), histogram based global thresholding; 2), morphological operation on the segmented binary output from step 1; 3), region growing process...
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