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The diphosphoryl derivative of the acyclic nucleotide phosphonate analog 9-[2-(phosphonomethoxy)ethyl]adenine (PMEA), found previously to weakly inhibit DNA pol δ/proliferating cell nuclear antigen, was studied as a substrate for pol α, δ, , and *. A comparison of the V max and K m for this derivative (PMEApp) and dATP demonstrated that the relative efficiency of the...
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