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Two enzymes possessing endonuclease activity were isolated from alfalfa seedlings. These enzymes were further purified by ammonium sulphate precipitation, DEAE cellulose, Heparin Sepharose and ssDNA-cellulose chromatography. One of them was an acid and the other a neutral nuclease with optimal activity at pH 5.5 and 7.0, respectively. Both enzymes showed a similar molecular weight of about 40kDa,...
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