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O‐GlcNAcylation of proteins regulates important cellular processes. A few reports noted that O‐GlcNAcylation exhibits cross‐talk with tyrosine phosphorylation. With an activity‐based microarray analysis of 256 tyrosine kinase peptide substrates, we found that phosphorylation of six peptides by Jak2 inhibits their subsequent O‐GlcNAcylation. However, O‐GlcNAcylation has no detectable effect on their...
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