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A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression...
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