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Large volume injection (LVI) has systematically been studied to improve LC–MS/MS sensitivity (signal-to-noise ratio, or S/N) and detection limits. The method of LVI was combined with on-line solid phase extraction (on-line SPE) and LC–MS/MS detection for analysis of compounds directly in plasma. It was demonstrated that LVI of plasma with on-line SPE-LC–MS/MS allows for improvement of sensitivity...
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