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The human fecal anaerobe Eubacterium ramulus is capable of degrading various flavonoids, including the flavone naringenin. The first step in the proposed degradation pathway is the isomerization of naringenin to the corresponding chalcone. Cell-free extracts of E. ramulus displayed chalcone isomerase activity. The enzyme from E. ramulus was purified to homogeneity. Its apparent molecular mass was...
Abstract. An NADH oxidase from the strictly anaerobic Eubacterium ramulus was purified to homogeneity. The enzyme is composed of two types of subunits with molecular masses of 40 and 30kDa. The molecular mass of the native enzyme is 450kDa according to gel filtration and PAGE analysis. Six to eight mol of FAD were found per mol of native enzyme. The NADH-specific enzyme was inhibited by N-bromosuccinimide...
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