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UDP-d-glucose: limonoid glucosyltransferase was purified from albedo tissues of navel orange (Citrus sinensis) cultivars, Frost and Newhall, by a combination of (NH 4 ) 2 SO 4 fractionation, UDP-glucuronic acid affinity chromatography and DEAE ion exchange HPLC. This procedure resulted in a 452-fold increase in enzyme purification. This enzyme catalysed the glucosylation...
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