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Stress and the nature of the transgene locus can affect transgene expression stability. These effects were studied in two, stably expressing, T6 populations of barley (Hordeum vulgare): bombardment-mediated, multi-copy lines with ubiquitin-driven bar and uidA or single-copy lines from Ds-mediated gene delivery with ubiquitin-driven bar alone. Imposing the environmental stresses, water and nutrient...
Methylation of plant promoters is often associated with transcriptional gene silencing, while methylation of the transcribed region is associated with post-transcriptional gene silencing. Promoter complexes that include the first untranslated exon and intron, such as maize ubiquitin1 and rice actin1, have been widely used in monocot transformation because they support higher levels of transient transgene...
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