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Using two-dimensional NMR spectroscopy, a complete 1 H resonance assignment has been obtained for the peptide magaining 2 recently isolated from Xenopus laevis. It is demonstrated that this peptide adopts an α-helical structure with amphiphilic character when dissolved in a mixture of trifluoroethanol (TFE) and H 2 O. The transition to the α-helical conformation occurs at very low...
A new NMR method is described for the generation of absorption mode two-dimensional NOE spectra of oligonucleotides in H 2 O solution. The method yields spectra that are free of baseline distortions with excellent suppression of the intense H 2 O resonance. The method is demonstrated for a sample of the dodecamer d(CGCGAATTCGCG) 2 . All exchangeable base protons are identified...
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