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To estimate allele frequency of single-nucleotide polymorphisms (SNPs) in pooled DNAs with secondary structures, an affinity capillary electrophoresis was developed using an allele-specific peptide nucleic acid probe modified with polyethylene glycol. This probe disrupted secondary structures of DNA analytes and hybridized to them during electrophoresis. Such DNA-binding capability allowed separation...
Quantitative SNP detection was demonstrated with an ACE using a PEG‐oligodeoxyribonucleotide block copolymer (PEG‐b‐ODN) as a probe in the presence of an EOF. The probe's PEG segment with large molecular weight and small polydispersity yielded a high resolution in the separation of a chemically synthesized 60‐base ssDNA (WT) and its single‐base‐substituted mutant (MT). A mixture of WT and MT was clearly...
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