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The ratio of the internucleotide dipolar coupling and the corresponding one-bond imino 15N-1H dipolar coupling provides a measure for the N···H/H-N distance ratio. Measurements were carried out for a dodecamer, d(CGCGAATTCGCG)2, in which a C-G and an A-T basepair were uniformly enriched in 15N. When assuming H-bonds to be perfectly linear, dipolar data indicate time-averaged hydrogen bond lengths...
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