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Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of 1Hα–13Cα(RMQ) is, on average, 1.3 ± 0.4 or 1.7 ± 0.6 times slower than the single-quantum relaxation rate of 13Cα(RC) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has...
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