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Two convenient colorimetric methods are described for the determination of nitric oxide (NO) concentration in neutral aqueous solutions. Both techniques take advantage of the fact that nitrosating and oxidizing intermediates are generated in the course of the NO/O 2 reaction. In the first method, 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) is oxidized to the corresponding cation radical,...
The use of electrochemical methods for the detection of various biological substances has provided real-time measurements giving insights into their roles in various physiological functions. The discovery that nitric oxide (NO) plays critical regulatory roles in a variety of physiological processes has prompted development of analytical techniques for the detection of this diatomic molecule in biological...
Since the discovery of nitric oxide (NO) as an endogenously formed radical, its effect on numerous physiological processes has been intensively investigated. Some studies have suggested NO to be cytotoxic while others have demonstrated it protective under various biological conditions. Though NO shows minimal cytotoxicity to a variety mammalian cell cultures, it does modulate the toxicity of some...
Reports on the kinetics of the autoxidation of aqueous nitric oxide are discussed. It is concluded that the correct rate law is -d[NO]/dt = 4k aq [NO] 2 [O 2 ] with k aq = 2 x 10 6 M -2 . s -1 at 25 o C and that a recent report of a rate law zero order in NO is incorrect.
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