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Mixed‐mode chromatography (MMC) is an interesting technique for challenging protein separation processes which typically combines adsorption mechanisms of ion exchange (IEC) and hydrophobic interaction chromatography (HIC). Adsorption equilibria in MMC depend on multiple parameters but systematic studies on their influence are scarce. In the present work, the influence of the pH value and ionic strengths...
Hydrophobic interaction chromatography (HIC) is often used for purifying proteins. The prediction of the adsorption equilibria in such systems is a difficult task as they depend on the pH value and the influence of the salts, which are added for controlling the solvent properties. This is especially tedious when salt mixtures are used, which can have positive or negative cooperative effects on the...
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