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Concentration and retention of a rod-shaped virus during tangential flow ultrafiltration (UF) was assessed to evaluate the potential of membrane-based downstream methods with advantages such as easy scale-up for industrial processes. A recombinant baculovirus of the non-spherical Autographa californica M nucleopolyhedrovirus (AcMNPV), vHSGFP, expressing egfp was filtered using polyethersulfone membranes...
Membrane-based virus filtration is used in biotechnological processes due to advantages such as easy scale-up but is currently lacking standardized validation protocols. Thus, ultrafiltration (UF) of a rod-shaped virus was assessed to contribute to the body of knowledge regarding the characterization of virus filtration. A recombinant baculovirus of Autographa californica M nucleopolyhedrovirus (AcMNPV),...
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