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The aims of this study were to compare the fertility of fresh and cryopreserved spermatozoa of the Japanese pearl oyster, Pinctada fucata martensii and to develop artificial fertilization methods using the cryopreserved spermatozoa. The optimal egg density for the fertilization test was found to be 1.0×10 5 eggs ml −1 seawater. When 1.0×10 5 eggs ml −1 seawater were...
To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular spermatozoa were diluted with cryopreservation diluent (10% methanol+18% fetal bovine serum+72% sea water), and dispensed into 0.25-mL straws...
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