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Purpose We aimed to validate and determine the possible application of transcervical insemination of frozen semen for improved breeding in guide dogs for the blind in Japan. Methods From February 2004 to March 2007, a total of 53 Labrador Retriever bitches, used for the breeding of guide dogs for the blind, were transcervically inseminated with frozen-thawed semen from 13 males by means of a cystoscope...
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