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The polyphenolic content, antioxidant and antibacterial activities of the ethanolic extracts of Piper betle leaf obtained from soxhlet (PBSx), sonication (PBSn) and maceration (PBMn) extraction methods were studied. GC–MS analysis was carried out to determine the variation in the phytoconstituents in these extracts. Whereas, thin-layer chromatography (TLC)–bioautography was conducted to localise,...
Trypsin inhibitor in cowpea Vigna unguiculata (CpTI) can be classified as the Bowman-Birk serine protease inhibitor and has been reported to be one of the antinutritional factors. Genes encoding these proteins have become an option to obtain insect-resistant transgenic plants. Monoclonal antibodies (mAbs) were generated using purified trypsin inhibitors from cowpea seeds to immunize the mice. One...
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