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Trehalose was pretreated by microwave heating with dilute acid to improve glucose yield and hydrogen production during dark-fermentation. An optimal glucose yield of 0.95 ± 0.08 g/g-trehalose was obtained when trehalose was pretreated by microwave heating with 1% dilute H 2 SO 4 for 25 min at 120 °C. The hydrolyzed trehalose was inoculated with HPB (hydrogen-producing bacteria) to...
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