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Cationic polymers have been receiving much attention as non-viral gene vectors. The aqueous mobile phase was optimized in combination with Shodex OHpak SB columns for size-exclusion chromatography (SEC) analysis of disulfide-containing poly(ethylene imine) (PEI) derivatives used in gene delivery. Addition of acetonitrile in mobile phase was shown to be able to suppress the hydrophobic interactions...
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