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Background Grana and starch are major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two-photon fluorescence (2PF) and second harmonic generation...
We demonstrate second-harmonic-generation (SHG) microscopic imaging on the polyhedral inclusion bodies (PIB) of nucleopolyhedrovirus in living cells. Due to a body-centeredcubic arrangement of polyhedrin trimers, these PIBs generate SHG with polarization anisotropy.
Optical higher harmonic generation, including second harmonic generation and third harmonic generation, leaves no energy deposition to its interacted matters due to an energy-conservation characteristic, providing the ''noninvasiveness'' nature desirable for biological studies. Combined with its nonlinearity, higher harmonic generation microscopy provides excellent three-dimensional (3D) sectioning...
Different crystal growth and quality information can be obtained through the simultaneous acquisition of transmitted and reflected second-harmonic-generation images, which is combined with multiphoton, multimodal nonlinear microscopy to study the optical property in lateral-epitaxially-overgrown GaN
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