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Many microRNAs (miRNAs) have been predicted from small RNA sequencing data, but little was experimentally verified due to the lack of effective methods. Here, we developed a simple and reliable dual gene expression cassette vector‐based method to verify predicted plant miRNAs. We cloned osa‐miR528 as a known miRNA, hvu‐miRX as a predicted miRNA and TaDREB3 open reading frame as a non‐miRNA into the...
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