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Embryonic stem cells (ESCs) and neural progenitor cells form three-dimensional (3D) colonies or spheres in vitro, and 3D sphere is reported to help maintaining the stemness of stem cells, but the effect of 3D sphere formation on cell reprogramming remains unknown. Here we examined whether 3D sphere culture have any impact on the differentiated cells. We cultured bladder cancer cell RT4 and non-cancerous...
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