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Dimerization is widely believed to be a requirement for the yeast transcriptional activator GCN4 to recognize its specific DNA target sites. We used the basic region (226-252) of the yeast transcriptional activator GCN4, both as a monomeric peptide and a disulfide-linked dimer to investigate the interaction of GCN4 peptides with the DNA target sites AP-1 and CRE. CD and ITC experiments suggest that...
Using isothermal titration calorimetry (ITC), we studied the thermodynamic parameters of the 15-mer duplex dsDNA [d(GAGATGACTCATCTC)]*[d(GAGATGAGTCATCTC)] formation from its two complementary single strands (S1 and S2) over a range of temperatures. The two complementary single strands d(GAGATGACTCATCTC) (herein called S1) and d(GAGATGAGTCATCTC) (herein called S2) containing palindromic sequences may...
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