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An extensive study is described to identify the most suitable fluorescent label in magnetic microsphere sedimentation arrays. The investigated fluorescent labels, commonly used in multiplex analysis, include organic dyes, (fluorescein, Alexa488, Cy5) fluorescent proteins (R-Phycoerythrin, Allophycocyanine, PBXL-3) polymer nanoparticles (FluoSpheres, PD-Pt) and semiconductor nanocrystals (Quantum dots)...
Magnetic microspheres are optically encoded by doping with three luminescent dyes. The combination of a fluorophore with a nanosecond decay profile and two phosphorescent Ruthenium metal ligand complexes with a microsecond decay profile generates a characteristic signature described by three features: bead brightness, luminescent decay time and dual lifetime referencing (DLR). The beads are identified...
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