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A trimeric β-lactosyl cluster based on 2-nitro-2-(hydroxymethyl)propane-1,3-diol was prepared using the trichloroacetimidate method. Kinetic studies showed that this cluster was an effective acceptor for rat-liver α-(2 → 3)-sialyltransferase. Its K M was comparable to those for monomeric lactose and N-acetyllactosamine acceptors, and its V max was 1% of that measured for...
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