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1. The effect of diclofenac (10-100 μM) on vanadate-induced contraction of rat uterus in calcium-free buffer containing EDTA and the modification of this response by pertussis toxin (50 μg/ml), Rp-cAMPS (10 μM), W-7 (10 and 60 μM), L-NMMA (10 and 100 μM) and D-NMMA (100 μM) has been assessed. The effects of sodium nitroprusside (10 μM-1mM), 3-morpholinosydnonimine (SIN-1; 0.1-100 μM), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one...
1. The effect of diclofenac (10-100 μM) on rat uterus contraction and its modification by ouabain (0.1 mM), amiloride (0.1 and 1 mM), ouabain (0.1 mM) plus amiloride (1 mM) and the replacement of sodium by choline have been assayed.2. Diclofenac produces dose-dependent relaxation of vanadate (0.3 mM)-induced contraction (EC 50 , 17.3 ± 1.8 μM). This effect is significantly reduced in...
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