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The polyphosphate operon of Escherichia coliwas overexpressed on a high copy plasmid under control of its native promoter. This operon contains the genes coding for polyphosphate kinase (PPK) and polyphosphatase (PPX), the enzymes responsible for polyphosphate synthesis and degradation, respectively. The plasmid was engineered for improved stability by addition of the parB locus. The polyphosphate...
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